You should start by learning INT 13h. That's what I'm trying to do right now. Try learning more about interrupts and filesystem. Which one do you want? FAT12? FAT16? FAT32? To load a program, you need filesystem support. By the way, I don't suggest an ISO unless you want to use it as a setup (installation for your OS). Right now, you need to focus on just booting with a floppy image.
Like Octocontrabass has said, you should look at the guides on the
OSDev Wiki.
That's my largest suggestion. I agree with Octocontrabass.